—
In terms of the development of gene sequencing in recent two years, "single cell sequencing" is undoubtedly the keyword. In recent years, research on single-cell sequencing has increased day by day. As of 2020, over 500 scientific papers have been published in Pubmed.Every plant, animal, and fungus is made up of an intricate amalgam of cells. Each cell type has its own unique function, life cycle, and reaction to its environment. These factors, in aggregate, inform how living things live, the way disease manifests, and why we die. Single cell sequencing is to sequence the genome from the single cell level, and apply the gene sequencing to the it, so as to identify the type and function of the cell, and the changes or mutations in the health or state of a particular cell. The conventional NGS-based genetic testing is based on genetic analysis of the entire cell population at the macroscopic level of a large number of cells. The technology has been widely used in the fields of neurobiology, germline transmission, organ growth, cancer biology, etc. in the scientific research industry.
Application in tumors
Single cell sequencing technology has been widely used in the field of scientific research, the most direct is the translational medical research for tumor immunity, concomitant diagnosis and medication guidance, including the evaluation of tumor immunotherapy, concomitant diagnosis, evaluation of efficacy, personalized medication guidance for tumors, identification of tumor and disease markers, and changes in cell groups.
For example, tumor neoantigen therapy is an application direction in which early single cell sequencing technology is combined with clinical applications. Tumor neoantigen is an individualized treatment plan. Each tumor cell has a specific antigen. From this, the researchers predict the neoantigen and carry out targeted treatment.
The presence and detection of new antigens is based on DNA rather than gene expression. This requires combining with single-cell sequencing to determine the expression intensity of new antigens to achieve rapid screening.
In another study, in the field of liquid biopsy, a Professor found that CTC (circulating tumor cell) detection can be used for early tumor screening and tumor metastasis detection. But CTC cells are heterogeneous, and different technical routes will capture different purity and subtypes of CTC, which is difficult to decide whether the tumor has been metastasized. The single-cell sequencing technology can detect and distinguish extremely small amounts of CTC, which is expected to explore the clinical value of CTC.
Single-cell proteomics also has application potential in tumor immunotherapy, including:
1. Through the detection of blood proteins, find biomarker combinations for early diagnosis of cancer and provide guidance for molecular targeted therapy.
2. Explore the cellular pathways, mechanisms of action of immunotherapy, and predict treatment response.
Application in others
Of course, the application of single-cell proteomics is not limited to the field of tumor immunity. It has been applied to basic research, drug development, and translational research in many fields such as immunology, stem cells, and vaccine research.
Single-cell sequencing can also be applied in the fields of microbial research, neuroscience, immunology, etc., for example:
1. Microbial analysis, such as new species identification, pathogen evolution, drug resistance detection, clinical diagnosis, etc.
2. Explore neuroscience analysis of neuronal cell subtypes and their onset mechanisms with Alzheimer's disease, Parkinson's disease, schizophrenia, depression and other diseases.
3. To accurately detect the genetic material of a single immune cell, analyze the role of a single immune cell rather than a tissue group of immune cells in the immune mechanism, and other fields of immunology research.
At CD Genomics, the company is dedicated to offering high-quality single-cell RNA sequencing, single-cell DNA sequencing and single-cell DNA methylation sequencing. Besides, CD Genomics's Single-Cell kit produces amplified DNA fragments suitable for Copy Number Variation (CNV) analysis using oligonucleotide aCGH or qPCR; SNP genotyping, mutation detection and sequencing.
Contact Info:
Name: Kikico Grace
Email: Send Email
Organization: CD Genomics
Address: 45-1 Ramsey Road
Phone: 6316197922
Website: https://www.cd-genomics.com/
Release ID: 88954595
Google
RSS