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Creative Biolabs, a biotech company specialized in providing custom biotechnology and pharmaceutical services that cover the full scope of biotechnology needs, announces that Creative Biolabs and In-Cell-Art (ICA) worked together to achieve a satisfactory result for a challenging antibody discovery project of Virbac, a global independent pharmaceutical company dedicated to animal health. As a part of Virbac’s in vitro program, this project focuses on discovering unique antibodies against a confidential target.In this collaboration, ICA took in charge of the DNA immunization stage, starting from DNA sequence optimization, synthesis, subcloning to ICA’s proprietary plasmid, amplification, purification, quality control, Nanotaxi® formulation, and the immunization of chosen animals.
Along with the immunizations been completed by ICA, Creative Biolabs was contracted by Virbac to discovery specific monoclonal antibodies through its Magic™ Antibody Discovery Platform. To meet the specific requirement of this project, Creative Biolabs adopted its advanced phage display technology using extracted RNA sent from ICA to construct an immune antibody library for specific antibody isolation. This one-stop service covers phage display library construction, biopanning, single clone validation, DNA sequencing and quality control to discover specific antibodies against the interested antigen in the monoclonal format. A simple sketch of this process is shown above.
Dong H. Chen, Chief Scientific Officer of Creative Biolabs, says “This is a good example to demonstrate the power of combining ICA’s Nanotaxi®/DNA immunization and Creative Biolabs’ Magic™ Antibody Discovery Platform which allows us to provide one-stop service for our customers to discover unique monoclonal antibodies with high specificity and affinity to their targets”.
Phage Display Library Construction and Screening
Creative Biolabs is able to construct immune antibody libraries and isolate monoclonal antibodies with high specificity and affinity. Antibody libraries are constructed by the genomic information coding for antibody variable domains, which can be derived from B cells of immune or naïve donors. High-quality libraries with the diversity of over 108 can be achieved. Through utilizing the appropriate biopanning strategy, high-affinity antibodies against the interested antigen can then be selected. It is one of the best approaches to raising monoclonal antibodies, especially from species that do not have hybridoma technology available.
Native™ Antibody Discovery Platform
Other than the advanced phage display technology, Creative Biolabs also provides the Native™ antibody discovery platform based on single B cell sorting. This unique technique allowing the discovery of naïve paired monoclonal antibodies with all primary antibody gene clusters from plasma and memory B cells of a wide range of species. Furthermore, the retrieved native antibodies have gone through in vivo affinity maturation which reveal extremely high in vivo specificity and affinity. It is now considered to be the best methods to raise high-affinity monoclonal antibodies for diagnostics and/or therapeutic antibody development.
Screening of Pre-made Libraries
For antigens that do not have an immune response in animals or cannot be applied to animals, Creative Biolabs can screen multiple pre-made naïve and synthetic libraries (e.g. human antibody library, premade single domain antibody library) to isolate specific antibodies against the antigen. On the basis of great biopanning strategies, state-of-the-art equipment, and extensive expertise, scientists of Creative Biolabs can tailor the most appropriate strategies to screen the interested libraries of the clients.
“These series of services by Creative Biolabs yielded a satisfactory number of monoclonal antibodies specific to the target for further evaluation at Virbac.”, says Bruno Pitard, head of Scientific Advisory Board and co-founder of In-Cell-Art.
About Creative Biolabs
Creative Biolabs is a professional service provider in developing highly specific, high-affinity monoclonal antibodies from all popular antibody production species, including rabbit, chicken, llama, camel, alpaca, cow, dog, mouse, rat, sheep, monkey, human, and even shark. Creative Biolabs also has years of experience in raising functional monoclonal antibodies against multiple-pass membrane proteins, haptens, and other challenging antigens.
Contact Info:
Name: Candy Swift
Email: Send Email
Organization: Creative Biolabs
Address: 45-1 Ramsey Road
Phone: 1-516-669-8109
Website: http://www.creative-biolabs.com
Release ID: 141480
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